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Short-term application of elevated temperature and drought influences the isotopic composition of winter wheat grains
Pernicová, Natálie ; Urban, Otmar ; Čáslavský, Josef ; Klem, Karel ; Trnka, Miroslav
The study aimed to determine the differences in carbon (δ13C) and nitrogen (δ15N) stable isotope ratios in grains of three winter wheat varieties grown under optimal and stress conditions. We found that the wheat variety has a significant effect on both δ13C and δ15N isotope ratios. Short-term (nine days) exposure to drought and high temperature during the heading or stem extension development phase significantly enhanced δ13C values, but only high temperature affected δ15N values. Enhanced δ15N values support the assumption that global warming causes a higher representation of the 15N isotope in plants. Moreover, significant interactive effects of temperature and water availability on the values of both isotopes were found implying that C and N metabolisms have been altered under the investigated stress conditions. We conclude that δ13C and δ15N isotope ratios of cereal grains are sensitive indicators of stress conditions, even short-term ones
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The effect of mycorrhizal nad saprotrophic fungi on yield properties and nitrogen uptake of tomato and leek plants
Kudláčková, Marta ; Albrechtová, Jana (advisor) ; Baláž, Milan (referee)
Currently looking for alternative approaches to crop production which would be in accord with sustainable development. The present thesis was aimed on testing of organic cultivation of tomato (Solanum lycopersicum L.) and leek (Allium porrum L.) by using amendment with organic maize biomass (Zea mays L.), mycorrhizal fungi and saprotrophic fungi. The effects of different combinations of microbial inoculations on nitrogen uptake, plant growth and yield were investigated in greenhouse conditions. Supplied 15 N-labelled organic matter was separated from the root system by a nylon mesh which permitted only fungal hyphae to pass through but not plant roots. In the first year experiments the treatments differed in the presence or absence of three factors: organic matter, saprotrophic fungus Agrocybe sp. and mycorrhizal fungus Glomus mosseae (Nicolaj & Gerd.) Gerd. & Trappe. Plant inoculation with Agrocybe sp. alone or together with G. mosseae increased plant growth of tomato in the presence of organic matter. Tomato yield were not increased significantly. Shoot dry weight of leek increased when plants were treated with mycorrhizal fungus G. mosseae and organic matter. Microbial inoculation did not influence nitrogen (15 N) uptake from the organic source. In following experiments, all treatments contained...
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